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PIK3CA(E545K)

Catalog Number: CR-R21145

Gene Symbol: PIK3CA(E545K)

Description: Anti-PIK3CA(E545K) Rabbit Polyclonal Antibody

Background: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The catalytic subunit is encoded by the PIK3CA gene. It phosphorylates PtdIns, PtdIns4P and

PtdIns(4,5)P2. The PIK3CA gene is one of the most frequently mutated genes in human cancers.

Immunogen: A synthetic peptide from theinternal region of PIK3CA(E545K) of human origin.

Tested applications: ELISA, WB, IHC, IP

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:100-1:1000

IHC: 1:10-1:100

Concentration: 0.2 mg/ml

Host: Rabbit

Clonality: Polyclonal

Purity: Purified from serum

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-PIK3CA(E545K) antibody recognizes PIK3CA(E545K) of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of PIK3CA wild type and E545K proteins. Equal amounts of purified PIK3CA(E545K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with anti-PIK3CA(E545K) rabbit polyclonal antibody (Cat. # 21145).