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Active Rab4

Catalog Number: CR-M26921

Gene Symbol: RAB4

Description: Anti-Active Rab4 Mouse Monoclonal Antibody

Background: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic.

Immunoprecipitation and Western blot:

Immunogen: Recombinant full length protein of active Rab4A.

Tested applications: IP, IHC

Recommended dilutions:

IP: 1 μg for 1~2 mg total cellular proteins

IHC: 1:50~100

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal

Isotype: IgG

Rab4A activation assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-active Rab4 monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel.

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rab4 antibody recognizes active Rab4 from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles