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Active Rab11

Catalog Number: CR-M26919

Gene Symbol: RAB11

Description: Anti-Active Rab11 Mouse Monoclonal Antibody

Background: Small GTPases of the Rab-family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.

Immunoprecipitation and Western blot:

Immunogen: Recombinant full length protein of active Rab11

Tested applications: IP, IHC

Recommended dilutions:

IP: 1 μg for 1~2 mg total cellular proteins

IHC: 1:50~100 dilution

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal

Isotype: IgG2a, IgG2b

Purity: Purified from ascites

Format: Liquid Storage buffer:

Rab11 activation assay. Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-active Rab11 monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rab11 antibody recognizes active Rab11 from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles