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Active Ral

Catalog Number: CR-M26913

Gene Symbol: RAL

Description: Anti-Active Ral Mouse Monoclonal Antibody

Background: The Ras-like small G proteins, RalA and RalB, are important components of Ras

signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell

migration, and cell proliferation.

Immunogen: Recombinant full length protein of active RalB.

Tested applications: IP, IHC

Recommended dilutions:1 μg for 1~2 mg total cellular proteins for IP;1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal Isotype: IgG

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Ral antibody

recognizes active RalA and RalB of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot

Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Ral monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalBrabbit polyclonal antibody (Cat. No. 21033).