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Active Rap1

Catalog Number: CR-M26912

Gene Symbol: RAP1

Description: Anti-Active Rap1 Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins. Immunogen: Recombinant full length protein of active Rap1.

Tested applications: IP, IHC

Recommended dilutions:1 μg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rap1 antibody recognizes active Rap1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot

Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1

polyclonal antibody (Cat. No. 21104).