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Active Ras

Catalog Number: CR-M26909

Gene Symbol: RAS

Description: Anti-Active Ras Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Ras belongs to

the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive.

GTP hydrolysis is aided by GTPase activating

proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange

factors (GEFs).

Immunogen: Recombinant full length protein of active H-Ras

Tested applications: IP, IHC

Recommended dilutions:1 μg for 1~2 mg total cellular proteins

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Ras antibody

recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation and Western blot:

IP/WB analysis of active Ras protein. Purified

recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-active Ras mouse monoclonal antibody (Cat. # 26909). After

SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody (Cat. #

26014).