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Active Rac1

Catalog Number: CR-M26903

Gene Symbol: RAC1

Description: Anti-Active Rac Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription.

GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive.

GTP hydrolysis is aided by GTPase activating

proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange

factors (GEFs).

Immunogen: Recombinant full length protein of active Rac1

Tested applications: IP, IHC

Recommended dilutions:

1 μg for 1~2 mg total cellular proteins Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal

Isotype: IgM

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation and Western blot:

Rac activation assay. MEF cells were treated

with (lane 2) or without (lane 1) PDGF. Cell

lysates were incubated with an anti-active Rac

monoclonal antibody (Cat # 26903) (top panel). The precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).

Immunohistochemistry:

Immunohistochemistry for the active Rac using

Anti-Active Rac1-GTP Mouse Monoclonal

Antibody [26903] shows Rac-GTP

immunolabeling (green) in combination with

cofilin (red) on brain tissue sections. The tissue

sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were

anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1:1000.