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CTNNB1 (D32N)

Catalog Number: CR-M26303

Gene Symbol: Beta-catenin, CTNNB, Catenin beta-1

Description: Anti-CTNNB1 (D32N) Mouse Monoclonal Antibody

Background: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteinsthat from adherens junctions, which are important for the establishment and maintenance ofepithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signaltransducer in the Wnt signaling pathway. Mutations ofCTNNB1 have been implicated in the pathogenesis of several cancers.

Immunogen: A synthetic peptide from theinternal region ofCTNNB1 which includes the mutation of D32N, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes D32N mutant, but not wild-type CTNNB1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinantCTNNB1 (D32N) and wildtype proteins.

Purified His-tagged CTNNB1 (D32N) protein (lane2) and corresponding wild-type protein(lane1) were blotted with anti-CTNNB1 (D32N) monoclonal antibody (Cat. #26303).

For research use only. Not for diagnostic or therapeutic applications.

Immunofluorescence:

Immunofluorescence of cells expressing

CTNNB1 proteins with anti-CTNNB1 (D32N) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left

column) or pCDNA3-GFP-CTNNB1 (D32N)

plasmid (right column), then fixed and stained

with anti-CTNNB1 (D32N) monoclonal antibody (Cat. #26303).