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KIT (P577-D579 DEL)

Catalog Number:CR-M26288

Gene Symbol: C-Kit; CD117; KIT; PBT; SCFR

Description: Anti-KIT (P577-D579 DEL) Mouse Monoclonal Antibody

Background: Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 orMast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activatingmutations in this gene are associatedwith gastrointestinal stromal tumors, testicular seminoma, mast celldisease, melanoma, acute myeloid leukemia,while inactivating mutations are associated with the genetic defect piebaldism.

Immunogen: A synthetic peptide from the internal region of KIT which includes the deletion of P577-D579, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes P577-D579 DEL mutant, but not wild-type KIT of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant

KIT (P577-D579 DEL) and wild-type proteins. Purified His-tagged KIT (P577-D579 DEL)protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-KIT(P577-D579 DEL) monoclonal antibody (Cat.#26288).

Immunofluorescence:

Immunofluorescence of cells expressing KIT proteins with anti-KIT (P577-D579 DEL)

antibody. HEK293T cells were transfected with pCDNA3-GFP-KIT (WT) plasmid (left column) or pCDNA3-GFP-KIT (P577-D579 DEL)

plasmid (right column), then fixed and stained with anti-KIT (P577-D579 DEL) monoclonal antibody (Cat. #26288).