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TP53(R175H)

Catalog Number: CR-M26072

Gene Symbol: TP53(R175H)

Description: Anti-TP53(R175H) Mouse Monoclonal Antibody

Background: TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a

tumor suppressor in many tumor types and

induces growth arrest or apoptosis depending on the physiological circumstances and cell type.

Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR),

adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.

Immunogen: A synthetic peptide from the internal region ofTP53 which includes the mutation of R175H, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:1000-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-TP53(R175H) antibody recognizes TP53(R175H) ofvertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant

TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wildtype protein(Cat.#10202) were blotted with anti-TP53(R175H) monoclonal

antibody(Cat.# 26072).

Immunofluorescence:

Immunofluorescence of cells expressing TP53 proteins with anti-TP53 (R175H) antibody.

HEK293T cells were transfected with

pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53 (R175H) plasmid (right column), then fixed and stained with anti- TP53 (R175H) monoclonal antibody (Cat. # 26072).

科博瑞（武汉）生物科技有限公司

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For research use only. Not for diagnostic or therapeutic applications.