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MEK1 (D67N)

Catalog Number:CR-M26224

Gene Symbol: MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1

Description: Anti-MEK1 (D67N) Mouse Monoclonal Antibody

Background: MEK1 (also known asextracellular signal-regulated kinases, ERKs)plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation anddifferentiation. Activated upon growth factors stimulation, MEK1 phosphorylatesMAPK3/ERK1 and MAPK1/ERK2, thus

activates the MAP pathway and regulatestranscription. Abnormal of the MEK1 protein,including point mutations, are implicated indiseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.

Immunogen: A synthetic peptide from the internal region of MEK1 which includes the mutation of D67N, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes D67N mutant, but not wild-type MEK1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant MEK1 (D67N) and wildtype proteins. Purified

His-tagged MEK1 (D67N) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with anti-MEK1 (D67N) monoclonalantibody (Cat. #26224).

Immunofluorescence:

Immunofluorescence of cells expressing MEK1 proteins with anti-MEK1 (D67N) antibody.

HEK293T cells were transfected withpCDNA3-GFP-MEK1 (WT) plasmid (leftcolumn) or pCDNA3-GFP-MEK1 (D67N)plasmid (right column), then fixed and stained with anti-MEK1 (D67N) monoclonal antibody (Cat. #26224).