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BRAF (D594G)

Catalog Number: CR-M26200

Gene Symbol: BRAF, BRAF1, RAFB1

Description: Anti-BRAF (D594G) Mouse Monoclonal Antibody

Background: BRAF belongs to a family of serine-threonine protein kinases.BRAF belongs to a family of serine-threonine protein kinases As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.

Immunogen: A synthetic peptide from the internal region of BRAF which includes the mutation of D594G, human origin.

Tested applications: ELISA, WB

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:500-1:1000

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes D594G mutant, but not wild-type BRAF of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant BRAF (D594G) and wildtype proteins. Purified His-tagged BRAF (D594G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-BRAF (D594G) monoclonal antibody (Cat. #26200).