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Ras(G12D)

Catalog Number: CR-M26036

Gene Symbol: RAS (G12D)

Description: Anti-Ras(G12D) Mouse Monoclonal Antibody

Background: K-Ras (G12D) is one type ofK-Ras missence mutations at codon 12, changed from GGU to GAU. K-Ras protein is a

membrane-associated GTPase signaling protein that regulates proliferation, differentiation,and

cell survival. K-Ras G12D mutation result in

decreased GTPase activity and constitutive

signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung

lesions.

Immunogen: Recombinant full length protein of K-Ras (G12D).

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA 1:100-1:5000

WB 1:100-1:1000

IHC 1:50-1:100

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal

Isotype: IgG1, IgG2a, IgG2b

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Ras(G12D) antibody

recognizes Ras(G12D) proteins of vertebrates. Storage Conditions: Store at -20°C. Avoid

freeze / thaw cycles

Western blot:

Western blot analysis of recombinant Ras WT and G12D proteins. Equal amount of purified

Ras WT (lane 1, cat. #10172) and G12D (lane 2, cat.# 10154) proteins were loaded and blotted

with anti-Ras G12D mouse monoclonal

antibody(Cat. # 26036) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat. # 21021).

Immunofluorescence:

Immunofluorescence of cell expressing

Ras(G12D) protein with anti-Ras(G12D)

antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(WT) plasmid (left column) or pCDNA3-GFP-Ras(G12D) plasmid (right

column), then fixed and stained with

anti-Ras(G12D) monoclonal antibody (Cat. # 26036).

For research use only. Not for diagnostic or therapeutic applications.