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IDH2 (R172K)

Catalog Number: CR-M26163

Gene Symbol: IDH2 (R172K)

Description: Anti-IDH2 (R172K) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH)catalyze the oxidative decarboxylation ofisocitrate to 2-oxoglutarate. The isocitrate andisopropylmalate dehydrogenases family hasthree members, IDH1, IDH2 and IDH3. IDH2plays a role in intermediary metabolism andenergy production. Defects in IDH2 are thecause of D-2-hydroxyglutaric aciduria type 2(D2HGA2). Somatic mosaic mutations of thisprotein have also been found associated to Ollier disease and Maffucci syndrome, and R172KIDH2 mutations do exist in diffusely infiltrative gliomas.

Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:100-1:1000

IHC: 1:10-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: recognizes R172K mutant, but not wild-type IDH2 of vertebrates.

Storage Conditions: Store at -20°C. Avoidfreeze / thaw cycles.

Western blot:

Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified

His-tagged IDH2 (R172K) (lane 2) and wildtype protein (lane 1) were blotted with anti-IDH2(R172K) monoclonal antibody (Cat. #26163).

Immunofluorescence:

Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172K) antibody.

HEK293T cells were transfected with pCDNA3-GFP- IDH2 (R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid orpCDNA3-GFP- IDH2 (R172S) plasmid,then fixed and stained with anti- IDH2 (R172K) monoclonal antibody (Cat. #26163)