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ATM (F858L)

Catalog Number: CR-M26141

Gene Symbol: ATM

Description: Anti-ATM (F858L) Mouse Monoclonal Antibody

Background: Ataxia telangiectasia mutated(ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteinsthat initiate activation of the DNA damagecheckpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers.

Immunogen: A synthetic peptide from the internal region of ATM which includes the mutation ofF858L, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:100-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes ATM (F858L), but not wild type ATM protein from vertebrates.

Storage Conditions: Store at -20°C. Avoidfreeze / thaw cycles.

Western blot:

Western blot analysis of recombinant ATM (F858L) and wildtype proteins. Purified

His-tagged ATM (F858L) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-ATM (F858L)

mouse monoclonal antibody (Cat. #26141).

For research use only. Not for diagnostic or therapeutic applications.

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (V769L) antibody.

HEK293T cells were transfected withpCDNA3-GFP-ATM (WT) plasmid (left column) or pCDNA3-GFP-ATM (F858L) plasmid (right column), then fixed and stained with anti-ATM (F858L) monoclonal antibody (Cat. #26141).