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B-Raf (N581S)

Catalog Number: CR-M26109

Gene Symbol: BRAF, BRAF1, RAFB1

Description: Anti-B-Raf (N581S) Mouse Monoclonal Antibody

Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.

Immunogen: A synthetic peptide from the internal region of Braf (N581S), human origin.

Tested applications: ELISA, WB, IHC, IP

Recommended dilutions:

ELISA 1:1000-1:5000

WB 1:500-1:2000

IHC 1:50-1:100

Concentration: 0.5 mg/ml

Host: Mouse

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes N581S mutated, but not wild-type B-Raf proteins fromvertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant B-raf proteins. Purified His-tagged B-Raf (N581S) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-B-Raf (N581S) monoclonal antibody (Cat. # 26109).

For research use only. Not for diagnostic or therapeutic applications.

Immunofluorescence:

Immunofluorescence of cells expressing B-Raf proteins with anti-B-Raf (N581S) antibody.

HEK293T cells were transfected withpCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-B-Raf (N581S) plasmid (right column), then fixed and stained with anti-B-Raf (N581S) monoclonal antibody (Cat. #26109).

科博瑞（武汉）生物科技有限公司

TEL：027-87615885 15972062685

Email：3636901710@qq.com

Web:www.coburybio.com

For research use only. Not for diagnostic or therapeutic applications.