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Ras (K117N)

Catalog Number: CR-M26468

Gene Symbol: H-Ras; K-Ras; N-Ras

Description: Anti-RAS (K117N) Mouse Monoclonal Antibody

Background: The K117N mutation ofKRasresults in an amino acid substitution at position146, from an alanine to a threonine. KRASencodes a protein that is a member of the smallGTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutivesignaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma,ductal carcinoma of the pancreas and colorectal carcinoma.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation ofK117N, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes K117N mutant, but not wild-type RAS of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant Ras (K117N) and wild-type proteins.

Purified His-tagged Ras (K117N) protein (lane 1) and corresponding wild-type protein (lane 2)were blotted with anti-Ras (K117N) monoclonal antibody (Cat. #26468).

Immunofluorescence:

Immunofluorescence of cells expressing KRas proteins with anti-KRas (K117N) antibody.

HEK293T cells were transfected withpCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRas (K117N) plasmid (right column), then fixed and stained with anti-KRas(K117N) monoclonal antibody (Cat. #26468).