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SMAD4 (R361H)

Catalog Number: CR-M26422

Gene Symbol: SMAD4, DPC4, JIP, MADH4, MYHRS

Description: Anti-SMAD4 (R361H) Mouse Monoclonal Antibody

Background: SMAD4 family member 4, alsoknown as SMAD4, is a protein that in human isencoded by the SMAD4 gene. It is often foundmutated in many cancers. It acts as a tumorsuppressor that functions in the regulation of the TGF-β signal transduction pathway, whichnegatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4alterations have been found inmultiploid colorectal cancer and pancreaticcarcinoma . It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdiseasejuvenilepolyposis syndrome (JPS).

Immunogen: A synthetic peptide from theinternal region of SMAD4 which includes the mutation of R361H, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes R361H mutant, but not wild-type SMAD4 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant SMAD4 (R361H) and wildtype proteins. PurifiedHis-tagged SMAD4 (R361H) protein (lane2) and corresponding wild-type protein (lane1) wereblotted with anti-SMAD4 (R361H) monoclonal antibody (Cat. #26422).

Immunofluorescence:

Immunofluorescence of cells expressing SMAD4 proteins with anti-SMAD4 (R361H) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4 (R361H) plasmidpCDNA3-GFP-SMAD4 (WT) plasmid, pCDNA3-GFP-SMAD4 (R361S) plasmid orpCDNA3-GFP-SMAD4 (R361C) plasmid, then fixed and stained with anti-SMAD4 (R361H) monoclonal antibody (Cat. #26422).