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EGFR (L858R)

Catalog Number: CR-M26082

Gene Symbol: EGFR/HER1/ERBB1

Description: Anti-EGFR (L858R) Mouse Monoclonal Antibody

Background: EGFR is a receptor tyrosinekinase binding ligands ofthe EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellularresponses. It activates at least 4 majordownstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT,PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer(LNCR).

Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858R, human origin.

Tested applications: ELISA, WB, IF, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:1000-1:5000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes EGFR (L858R), but not wild type EGFR from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant EGFR (L858R) and wildtype proteins. PurifiedHis-tagged EGFR (L858R) protein (amino acid702-987, lane 2, Cat. # 10230) and corresponding wild type protein (lane 1, Cat. # 10210) wereblotted with anti-EGFR (L858R) mousemonoclonal antibody (Cat. # 26082) . Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (L858R) antibody.

HEK293T cells were transfected withpCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (L858R) plasmid (right column), then fixed and stained with anti- EGFR (L858R) monoclonal antibody (Cat. #26082).

Immunohistochemistry:

Immunohistochemical analysis of

paraffin-embedded human NSCLC tissue with anti-EGFR (L858R) monoclonal antibody (Cat. #26082). Tissue samples were fixed with

formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heatmediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100)overnight at 4°C. A HRP-conjugated Goatanti-mouse IgG (dilution 1:50) was used as secondary antibody.