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IDH2 (R172S)

Catalog Number: CR-M26408

Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Description: Anti-IDH2 (R172S) Mouse Monoclonal Antibody

Background: Isocitrate dehydrogenase (IDH)catalyze the oxidative decarboxylation ofisocitrate to 2-oxoglutarate. The isocitrate andisopropylmalate dehydrogenases family hasthree members, IDH1, IDH2 and IDH3. IDH2plays a role in intermediary metabolism andenergy production. Defects in IDH2 are thecause of D-2-hydroxyglutaric aciduria type 2(D2HGA2). Somatic mosaic mutations of thisprotein have also been found associated to Ollier disease and Maffucci syndrome, and R172SIDH2 mutations do exist in diffusely infiltrative gliomas.

Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172S, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:100-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: recognizes R172S mutant, but not wild-type IDH2 of vertebrates.

Storage Conditions: Store at -20°C. Avoidfreeze / thaw cycles.

Western blot:

Western blot analysis of recombinant IDH2 (R172S) and wildtype proteins. PurifiedHis-tagged IDH2 (R172S) (lane 1) andcorresponding wild-type IDH2 protein (lane 2)were blotted with anti-IDH2 (R172S) monoclonal antibody (Cat. #26408).

Immunofluorescence:

Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172S) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH2 (R172S) plasmid, pCDNA3-GFP-IDH2 (WT) plasmid, pCDNA3-GFP-IDH2 (R172G) plasmid, pCDNA3-GFP-IDH2 (R172K) plasmid,pCDNA3-GFP-IDH2 (R172W) plasmid or pCDNA3-GFP-IDH2 (R172H) plasmid, then fixed and stained with anti-IDH2 (R172S) monoclonal antibody (Cat. #26408)