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BRCA2 (352-643)

Catalog Number: CR-M26364-1

Gene Symbol: BRCA2, BRCC2, FAD1,FCD, BROVCA2, PNCA2

Description: Anti-BRCA2 (352-643) Mouse Monoclonal Antibody

Background: BRCA2 is a human tumorsuppressor gene. Its protein, also called bythe synonym breast cancer type 2 susceptibility protein, is responsible for repairing DNA.Mutations of BRCA2 can cause an increased risk of breast cancer. In addition to breast cancer in

men and women, mutations in BRCA2 also lead to an increased risk of ovarian,Fallopiantube, prostate, and pancreatic cancers, as well as malignant melanoma .

Immunogen: Purified BRCA2 (352-643) protein, human origin.

Tested applications: ELISA, WB, IF, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: RecognizesBRCA2(352-643) of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant BRCA2 proteins.

Purified His-tagged BRCA2 (352-643) protein (lane1), His-tagged BRCA2 (1475-1748)protein(lane2) ，His-tagged BRCA2 (2213-2543) protein(lane3) and His-tagged BRCA2(2793-3030) protein (lane4) were blotted with anti-BRCA2 (352-643) monoclonal antibody (Cat. #26364-1).

Immunofluorescence:

Immunofluorescence of cells expressing

BRCA2（352-643）protein with anti-BRCA2 (352-643) antibody. HEK293T cells wastransfected with pCDNA3-GFP-BRCA2(352-643) plasmid, then fixed and stained with anti-BRCA2 (352-643) monoclonal antibody (Cat. #26364-1).