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RHEB (Y35N)

Catalog Number: CR-M26338

Gene Symbol: RHEB

Description: Anti-RHEB (Y35N) Mouse Monoclonal Antibody

Background: RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteinsoriginally found to be rapidly induced bysynaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growthfactor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway.The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as asensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1)complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity.

Immunogen: A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes Y35N mutant, but not wild-type RHEB of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant RHEB (Y35N) and wildtype proteins. PurifiedHis-tagged RHEB (Y35N) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-RHEB (Y35N) monoclonal antibody (Cat. #26338).

Immunofluorescence:

Immunofluorescence of cells expressing RHEB proteins with anti-RHEB (Y35N) antibody.

HEK293T cells were transfected withpCDNA3-GFP-RHEB (WT) plasmid (leftcolumn) or pCDNA3-GFP-RHEB (Y35N)plasmid (right column), then fixed and stained with anti-RHEB (Y35N) monoclonal antibody (Cat. #26338).