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NFE2L2 (L30F)

Catalog Number: CR-M26332

Gene Symbol: NFE2L2, NRF2

Description: Anti-NFE2L2 (L30F) Mouse Monoclonal Antibody

Background: NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress. is part of a complex of proteins that form adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells is part of a complex of proteins that form adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells

Immunogen: A synthetic peptide from the internal region of NFE2L2 which includes the mutation of L30F, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes L30F mutant, but not wild-type NFE2L2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant NFE2L2 (L30F) and wildtype proteins. Purified His-tagged NFE2L2 (L30F) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-NFE2L2 (L30F) monoclonal antibody (Cat. #26332).

Immunofluorescence:

Immunofluorescence of cells expressing NFE2L2 proteins with anti-NFE2L2 (L30F) antibody. HEK293T cells were transfected with pCDNA3-GFP-NFE2L2 (WT) plasmid (left column) or pCDNA3-GFP-NFE2L2 (L30F) plasmid (right column), then fixed and stained with anti-NFE2L2 (L30F) monoclonal antibody (Cat. #26332).