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GNAQ (Q209R)

Catalog Number: CR-M26330

Gene Symbol: GNAQ, CMC1, G-ALPHA-q, GAQ, SWS

Description: Anti-GNAQ (Q209R) Mouse Monoclonal Antibody

Background: GNAQ is a protein that in humans encoded by the GNAQ gene. The GNAQ protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospholipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation.is part of a complex of proteins that form adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells is part of a complex of proteins that form adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells

Immunogen: A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209R, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes Q209R mutant, but not wild-type GNAQ of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant GNAQ (Q209R) and wildtype proteins. Purified His-tagged GNAQ (Q209R) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-GNAQ (Q209R) monoclonal antibody (Cat. #26330).

Immunofluorescence:

Immunofluorescence of cells expressing GNAQ proteins with anti-GNAQ (Q209R) antibody. HEK293T cells were transfected with pCDNA3-GFP-GNAQ (WT) plasmid (left column) or pCDNA3-GFP-GNAQ (Q209R) plasmid (right column), then fixed and stained with anti-GNAQ (Q209R) monoclonal antibody (Cat. #26330).