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GNAQ (Q209P)

Catalog Number: CR-M26329

Gene Symbol: GNAQ, CMC1, G-ALPHA-q, GAQ, SWS

Description: Anti-GNAQ (Q209P) Mouse Monoclonal Antibody

Background: GNAQ is a protein that in humans encoded by the GNAQ gene. The GNAQ protein, an alpha subunit in the Gq class, couples aseven-transmembrane domain receptor toactivation of phospholipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation.

Immunogen: A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209P, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes Q209P mutant, but not wild-type GNAQ of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant GNAQ (Q209P) and wildtype proteins. PurifiedHis-tagged GNAQ (Q209P) protein (lane2) and corresponding wild-type protein (lane1) wereblotted with anti-GNAQ (Q209P) monoclonal antibody (Cat. #26329).

Immunofluorescence:

Immunofluorescence of cells expressing GNAQ proteins with anti-GNAQ (Q209P) antibody.

HEK293T cells were transfected with pCDNA3-GFP-GNAQ (Q209P) plasmid , pCDNA3-GFP-GNAQ (WT) plasmid, pCDNA3-GFP-GNAQ (Q209L) plasmid or pCDNA3-GFP-GNAQ (Q209R) plasmid,then fixed and stained with anti-GNAQ (Q209P) monoclonal antibody (Cat. #26329).