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CTCF (K344E)

Catalog Number: CR-M26324

Gene Symbol: CTCF; MRD21

Description: Anti-CTCF (K344E) Mouse Monoclonal Antibody

Background: Transcriptional repressor CTCF also known as 11-zinc fingerprotein or CCCTC-binding factor isatranscription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptionalregulation, insulator activity, recombination and regulation of chromatin architecture.

Immunogen: A synthetic peptide from the internal region of CTCF which includes the mutation ofK344E, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:5000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes K344E mutant, but not wild-type CTCF of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant CTCF (K344E) and wild-type proteins.

Purified His-tagged CTCF (K344E) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-CTCF (K344E)monoclonal antibody (Cat. #26324).

Immunofluorescence:

Immunofluorescence of cells expressing CTCF proteins with anti-CTCF (K344E) antibody.

HEK293T cells were transfected withpCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (K344E) plasmid (rightcolumn), then fixed and stained with

anti-CTCF (K344E) monoclonal antibody (Cat.#26324).