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BRCA1 (C61G)

Catalog Number: CR-M26319

Gene Symbol: BRCA1, BRCC1, IRIS, PNCA4, PPP1R53, PSCP

Description: Anti-BRCA1 (C61G) Mouse Monoclonal Antibody

Background: BRCA1 is normally expressed in cells of breast and other tissues, where it helps repair damaged DNA or destroy cells if DNA cannot be repaired. It is involved in the repair of chromosomal damage with an important role in the error-free repair of DNA double-strand breaks. If BRCA1 itself is damaged by a BRCA1 mutation, damaged DNA is not repaired properly, and this increases the risk for breast cancer. is part of a complex of proteins that form adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells is part of a complex of proteins that form adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells

Immunogen: A synthetic peptide from the internal region of BRCA1 which includes the mutation of C61G, human origin.

Tested applications: ELISA, WB, IHC

Recommended dilutions:

ELISA: 1:1000-1:2000

WB: 1:500-1:1000

IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Purity: Purified from ascites

Format: Liquid

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes C61G mutant, but not wild-type BRCA1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant BRCA1 (C61G) and wildtype proteins. Purified His-tagged BRCA1 (C61G) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-BRCA1 (C61G) monoclonal antibody (Cat. #26319).

Immunofluorescence:

Immunofluorescence of cells expressing BRCA1 proteins with anti-BRCA1 (C61G) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRCA1 (WT) plasmid (left column) or pCDNA3-GFP-BRCA1 (C61G) plasmid (right column), then fixed and stained with anti-BRCA1 (C61G) monoclonal antibody (Cat. #26319).