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货号：CR-M26901
Anti-Active Gαi Mouse Monoclonal Antibody
规格：30ul
30ul
库存：500
500
免疫原：Immunogen: Recombinant full length protein ofactive Gαi1
Immunogen: Recombinant full length protein ofactive Gαi1
应用：Tested applications: IP, IHC
Tested applications: IP, IHC
浓度：Concentration: 1 mg/ml
Concentration: 1 mg/ml
同种型：Isotype: IgG1
Isotype: IgG1
货期：现货
现货
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Active Gαi
Catalog Number: CR-M26901
Gene Symbol: Gnai
Description: Anti-Active Gαi Mouse Monoclonal Antibody
Background: Heterotrimeric G proteins are critical
cellular signal transducers. Gαi represents one
sub-family of G proteins that could mediate the
inhibition of adenylyl cyclases. Other biochemical
i proteins are and physiological functions of Gα
being explored.
Immunogen: Recombinant full length protein ofactive Gαi1
Tested applications: IP, IHC
Recommended dilutions:
1 µg for 1~2 mg total cellular proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG1
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4,
150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Gαi antibody
i1, Gαi2, and Gαi3 of recognizes active Gα
vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze /
thaw cycles
cellular signal transducers. Gαi represents one
sub-family of G proteins that could mediate the
inhibition of adenylyl cyclases. Other biochemical
i proteins are and physiological functions of Gα
being explored.
Immunogen: Recombinant full length protein ofactive Gαi1
Tested applications: IP, IHC
Recommended dilutions:
1 µg for 1~2 mg total cellular proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG1
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4,
150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Gαi antibody
i1, Gαi2, and Gαi3 of recognizes active Gα
vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze /
thaw cycles
Immunoprecipitation/Western blot
Gαi activation assay. A. CHO cells were transfected
with wild-type Gαi1 (lanes 1 and 2) or constitutively active
Gαi1-Q204L (lane 3). Cell lysates were treated with GDP
(lane 1) or GTPγS (lane 3). Lysates were then incubated
with an anti-active Gαi monoclonal antibody (Cat. No.
26901) (top panel). The precipitated active Gαi was
immunoblotted with an anti- Gαi monoclonal antibody (Cat.
No. 26003). The bottom panel shows the Western blot with
anti- Gαi monoclonal antibody (Cat.No. 26003) of the cell
lysates. B. HEK293 cells stably expressing human m2
mAChR were treated with (lane 2) or without (lane 1)
carbachol. Cell lysates were then incubated with an
anti-active Gαi monoclonal antibody (Cat. No. 26901) (top
panel). The precipitated active Gαi was immunoblotted
with an anti- Gαi rabbit polyclonal antibody (Cat. No.
21006). The bottom panel shows the Western blot with
anti-tubulin of the cell lysates..
with wild-type Gαi1 (lanes 1 and 2) or constitutively active
Gαi1-Q204L (lane 3). Cell lysates were treated with GDP
(lane 1) or GTPγS (lane 3). Lysates were then incubated
with an anti-active Gαi monoclonal antibody (Cat. No.
26901) (top panel). The precipitated active Gαi was
immunoblotted with an anti- Gαi monoclonal antibody (Cat.
No. 26003). The bottom panel shows the Western blot with
anti- Gαi monoclonal antibody (Cat.No. 26003) of the cell
lysates. B. HEK293 cells stably expressing human m2
mAChR were treated with (lane 2) or without (lane 1)
carbachol. Cell lysates were then incubated with an
anti-active Gαi monoclonal antibody (Cat. No. 26901) (top
panel). The precipitated active Gαi was immunoblotted
with an anti- Gαi rabbit polyclonal antibody (Cat. No.
21006). The bottom panel shows the Western blot with
anti-tubulin of the cell lysates..