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Active Sar1

Catalog Number: CR-M26916

Gene Symbol: SAR1

Description: Anti-Active Sar1 Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of

these small GTPases. Sar1 functions as amolecular switch to control protein–protein andprotein–lipid interactions that direct vesicle budding from the ER.

Immunogen: Recombinant full length protein of active Sar1a.

Tested applications: IP, IHC

Recommended dilutions:1 μg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal Isotype: IgG

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Sar1 antibody recognizes active Sar1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot

Sar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTP□S (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No.

26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090).