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Active Gαo

Catalog Number: CR-M26907

Gene Symbol: Gnao

Description: Anti-Active Gαo Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one

sub-family of G proteins that could mediate the

inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are

being explored.

Immunogen: Recombinant full length protein of active Gαo

Tested applications: IP, IHC

Recommended dilutions:1 μg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Clonality: Monoclonal

Isotype: IgG1

Purity: Purified from ascites

Format: Liquid Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Gαo antibody recognizes active Gαo of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot

Gαo activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti-Gαo polyclonal antibody (Cat. No. 21015).