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周一至周日 ：8:00-18:00
货号：CR-W82601
Rab3A Pull-Down Activation Assay Kit
规格：30 Assays
30 Assays
库存：3000
3000
免疫原：Recombinant full length active Gα13 protein
Recombinant full length active Gα13 protein
应用：IP, IHC
IP, IHC
浓度：1 mg/ml
1 mg/ml
同种型：IgG1, IgG2b
IgG1, IgG2b
货期：现货
现货
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Rab3A Pull-Down Activation Assay Kit
Cat. # CR-W82601
Background
Rab3A is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles Currently there is no direct assay to measure the activation of Rab3 GTPases.
The Rab3A Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab3-GTP, but not Rab3-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Currently there is no direct assay to measure the activation of Rab3 GTPases.
Assay Principle
The Rab3A Activation Assay Kit uses configuration-specific anti-Rab3-GTP Mouse monoclonal antibody to measure Rab3-GTP levels in cell extracts or in vitro GTPγS loading Rab3 activation assays. Anti-Rab3-GTP mouse monoclonal antibody is first incubated with cell lysates containing Rab3-GTP. Next, the GTP-bound Rab3 is pulled down by protein A/G agarose. Finally, the precipitated Rab3-GTP is detected through immunoblot analysis using Anti-Rab3A Rabbit Polyclonal Antibody.